Core Facilities and Platforms

Various technology platforms and core facilities are available at the Meakins-Christie Laboratories and the RI-MUHC.

RI-MUHC Technology Platforms

At the RI-MUHC, a number of technology platforms are available to the research community:
– Containment Level 3 (CL3) Platform
– Drug Discovery Platform
– Histopathology Platform
– Immunophenotyping Platform
– Molecular Imaging Platform
– Proteomics Platform
– Small Animal Imaging Labs (SAIL)
– Bioinformatics Platform
– RI-MUHC Biobank

Biobanks

A key aspect to translational research is access to patient-derived samples and tissues. The Meakins, together with RECRU or other RI-MUHC programs, is actively involved in the establishment and maintenance of various biobanks:

Biobanks at the meakins-christie laboratories
Asthma

Asthma

The Severe Asthma Program banks asthmatic tissues and bronchoscopic biopsies in conjunction with the Quebec Respiratory Health Network of the FRQS.

Cystic Fibrosis

Cystic Fibrosis

The CF group collects sputum, nasal epithelial cells, and serum from CF patients at baseline and during exacerbations in collaboration with the CF clinic.

Tuberculosis

Tuberculosis

The TB group collects serum before and during treatment and stool samples to assess the gut microbiome.

Lung

Lung

A collaboration with Thoracic Surgery and Pathology collects fresh and fixed lung and diaphragm tissues and blood from patients undergoing lung resection procedures.

Muscle

Muscle

Diaphragm tissues and limb muscle biopsies from critically-ill patients in the intensive care unit.

Pulmonary Fibrosis

Pulmonary Fibrosis

A biobank linked to well characterized patient cohorts with different forms of autoimmune disease-associated interstitial-lung diseases.

Meakins-Christie Core Facilities

Smooth Muscle Biomechanics and Respiratory System Mechanics

A small animal pulmonary function laboratory is supervised by Dr. James Martin and a smooth muscle mechanics laboratory supervised by Dr. Anne-Marie Lauzon. Additional details available on Dr. Anne-Marie Lauzon’s webpage.

Equipment and Techniques Available: Measurement of respiratory system mechanics (Flexivent), Allergen and methacholine challenge testing (mice, rats and larger rodents), Measurement of isometric and isotonic shortening of both smooth and skeletal muscle (In vitro motility and laser trap assays, and muscle strip force-velocity setup).

Skeletal Muscle Biomechanics

Skeletal Muscle Biomechanics

Additional details available on Dr. Basil Petrof’s webpage.

Equipment and Techniques Available: In vitro organ bath preparation to determine diaphragmatic force generating capacity.

Flow Cytometry

Flow Cytometry

The Meakins Flow Cytometry core is managed by Dr. Irah King. Training for all equipment is mandatory. 

Equipment Available: FACSCanto II with autoloader, ImageStream®X Mark II Imaging Flow Cytometer, BD’s  LSR II and Accuri C6 flow cytometers.

Imaging

Imaging

The Meakins provides training on all microscopes.

Equipment available: Multihead microscope for teaching purposes, Olympus and Zeiss fluorescence microscopes, Morphometry (software and hardware), Inverted fluorescence microscopy for live cell imaging and Ca2+ experiments, Arcturus XT laser capture microdissection system, Zeiss LSM700 confocal microscope, and fluorescence slide scanner.

Molecular Pathology

Molecular Pathology

Teaching and hands-on training for students and fellows is provided.

Equipment available: Leica cryostat, several Leica Microtomes, a Roche benchmark Ultra for Automated immunocytochemistry / In situ hybridization slide staining, Arcturus XT laser capture microdissection system, Droplet Digital PCR.

Centre for Innovative Medicine

The Centre for Innovative Medicine (CIM) provides researchers with access to a full range of clinical research services in a state-of-the-art environment dedicated to clinical research, including: muscle biopsies, pulmonary function testing, sleep laboratories, a cardiopulmonary physiology exercise lab, a medical imaging platform (CT, MRI, X-ray, US), a surgery facility including a bronchoscopy room.